Correction: Alkaline versatile peroxidase by directed evolution
نویسندگان
چکیده
منابع مشابه
Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase.
The VPs (versatile peroxidases) secreted by white-rot fungi are involved in the natural decay of lignin. In the present study, a fusion gene containing the VP from Pleurotus eryngii was subjected to six rounds of directed evolution, achieving a level of secretion in Saccharomyces cerevisiae (21 mg/l) as yet unseen for any ligninolytic peroxidase. The evolved variant for expression harboured fou...
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There are errors in the Funding section. The complete, correct funding information is as follows: This work was funded by the Commission of the European Communities through the PEROXICATS (KBBE-2010-4-265397, Novel and more robust fungal peroxidases as industrial biocatalysts) and INDOX (KBBE-2013-7-613549, "Optimized oxidoreductases for medium and large scale industrial biotransformations") pr...
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Biotechnology applications of horseradish peroxidase (HRP) would benefit from access to tailor-made variants with greater specific activity, lower K(m) for peroxide, and higher thermostability. Starting with a mutant that is functionally expressed in Saccharomyces cerevisiae, we used random mutagenesis, recombination, and screening to identify HRP-C mutants that are more active and stable to in...
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The stunning array of features and functions exhibited by proteins in nature should convince most scientists of the power of evolutionary design processes. Natural selection acting on populations over long periods of time has generated a vast number of proteins ideally suited to their biological functions. When we try to recruit these remarkable molecular machines for new taskssfrom serving as ...
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To develop a simple, speedy, economical and widely applicable method for multiple-site mutagenesis, we have substantially modified the Quik-Change Site-Directed Mutagenesis Kit protocol (Stratagene, La Jolla, CA). Our new protocol consists of (i) a PCR reaction using an in vitro technique, LDA (ligation-during-amplification), (ii) a DPN:I treatment to digest parental DNA and to make megaprimers...
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ژورنال
عنوان ژورنال: Catalysis Science & Technology
سال: 2016
ISSN: 2044-4753,2044-4761
DOI: 10.1039/c6cy90107g